Sequencing Core Facility

RNA Sequencing

The combination of NGS and polyA selection or ribo-depletion of total RNA has revolutionized gene expression analysis. RNA-seq at the CGT including polyA-selected mRNA-seq, whole transcriptome analysis, tag profiling and small RNA discovery allows for the exploration of functional genomics in model organisms and agricultural samples.

  • Identity tissue-specific gene expression patterns at high resolution
  • Explore differential expression across disease conditions
  • Discover novel splice variants, SNVs and insertion/deletions
  • Interrogate long non-coding RNAs as well as small RNAs


Services Deliverables:

  • Quantity and quality confirmation of total RNA
  • Whole transcriptome stranded sample prep
  • Illumina NextSeq/NovaSeq NGS technology
  • 4-6 week turnaround time
  • Fastq files and QC reports
  • Consultation with the Center for Genetic Epidemiology and Statistical Genetics


Volume discounts may apply. Custom designs, extractions and analysis are also available. For more information or to request a quote, contact us at:

CGTservices@med.miami.edu
305-243-5CGT

We welcome all inquiries and look forward to serving your research needs!