Sequencing Core Facility

RNA Sequencing

The combination of NGS and polyA selection or ribo-depletion of total RNA has revolutionized gene expression analysis. RNA-seq at the CGT including polyA-selected mRNA-seq, whole transcriptome analysis, tag profiling and small RNA discovery allows for the exploration of functional genomics in model organisms and agricultural samples.

  • Identity tissue-specific gene expression patterns at high resolution
  • Explore differential expression across disease conditions
  • Discover novel splice variants, SNVs and insertion/deletions
  • Interrogate long non-coding RNAs as well as small RNAs


Services Deliverables:

  • Quantity and quality confirmation of total RNA
  • Whole transcriptome stranded sample prep
  • Illumina NextSeq/HiSeq NGS technology
  • 4-6 week turnaround time
  • Fastq files and QC reports
  • Consultation with the Center for Genetic Epidemiology and Statistical Genetics


Pricing:

  • Gene expression profiling – $359/sample
    25 million single-end 75 base reads
  • Gene expression profiling – $489/sample
    25 million paired-end 75 base reads
  • Gene expression with noncoding – $495/sample
    40 million single-end 100 base reads
  • Gene expression with noncoding & splicing – $762/sample
    40 million paired-end 100 base reads


All pricing covers total RNA through raw data generation and assumes a 24 sample submission. Additional volume discounts may apply. Custom designs, extractions and analysis are also available. For more information or to request a quote, contact us at:

CGTservices@med.miami.edu
305-243-5CGT

We welcome all inquiries and look forward to serving your research needs!