HIHG Centers : Center for Molecular Genetics

Center for Molecular Genetics

The Center for Molecular Genetics at the John P. Hussman Institute for Human Genomics is part of a world-class research program for the discovery and characterization of human disease associated genes utilizing innovative molecular genetic, biochemical, and cell biological methods. Increasingly, human genetics relies on the convergence of multiple layers of genomic information. In the study of disease genes, it is crucial to understand a gene’s biological mechanisms and ultimately its potential role in causing disease. Understanding the molecular consequences of such genetic variation is essential for subsequent disease modeling and the development of therapeutic approaches. Faculty members working in the Center consist of scientists with expertise in molecular genetics, genomics, functional genomics, and epigenetics as they relate to human disease. The CHGM provides state-of-the-art molecular genetic know-how and technology for the discovery and molecular characterization of human disease genes and encompasses two Divisions, as well as, an induced pluripotent stem cell (iPSC) and imaging core facilities.

The Center is comprised of two divisions:

Division for Functional Genomics, Division Director
Gaofeng Wang, Ph.D. GWang@med.miami.edu

The Division for Functional Genomics focuses on genomic sciences as they relate to human disease. Utilizing the vast amount of data produced by the various -omics projects, such as genome sequencing, single cell and single nuclei RNAseq, and chromatin structure analysis (for example, chromatin conformation analysis), this division aims to describe gene functions and interactions. These studies include the functional analysis of the impact of coding and non-coding genomic variants using state-of-the-art approaches, including genome editing and gene perturbation studies, gene expression analysis, epigenetic and chromatin structure analysis, RNA metabolism, and protein functional studies.

Division of Human Disease Modeling, Division Director
Katherina Walz, Ph.D. Kwalz@med.miami.edu

The HIHG mouse Behavioral Core (MBC) provides planning, execution, and analysis of behavioral experiments examining activity, arousal, coordinated movement, learning and memory, anxiety, depression, seizure susceptibility, reward/reinforcement, aggression and motor abilities in mice. Additional tests can potentially be developed on a case-by-case basis. The Mouse Behavioral Core also offers several rodent surgical services and assistance in IACUC protocol preparation.

The MBC is located in the BRB building, within the barrier rodent vivarium facility. The HIHG has already assigned 4 suites for housing mice in an exclusive way. We can house more than 3,500 mice if necessary. Facilities for rodents include full barrier, air shower upon entrance and exit, individually ventilated racks, full cage washing and autoclaving facilities, set up for automatic watering, ample procedure space, necropsy facilities, and isolation areas.